RGD Reference Report - Growth cone morphology and spreading are regulated by a dynamin-cortactin complex at point contacts in hippocampal neurons. - Rat Genome Database

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Growth cone morphology and spreading are regulated by a dynamin-cortactin complex at point contacts in hippocampal neurons.

Authors: Kurklinsky, S  Chen, J  McNiven, MA 
Citation: Kurklinsky S, etal., J Neurochem. 2011 Apr;117(1):48-60. doi: 10.1111/j.1471-4159.2011.07169.x. Epub 2011 Feb 9.
RGD ID: 10047315
Pubmed: PMID:21210813   (View Abstract at PubMed)
PMCID: PMC3278041   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1471-4159.2011.07169.x   (Journal Full-text)

Neuronal growth cone (GC) migration and targeting are essential processes for the formation of a neural network during embryonic development. Currently, the mechanisms that support directed motility of GCs are not fully defined. The large GTPase dynamin and an interacting actin-binding protein, cortactin, have been localized to GCs, although the function performed by this complex is unclear. We have found that cortactin and the ubiquitous form of dynamin (Dyn) 2 exhibit a striking co-localization at the base of the transition zone of advancing GCs of embryonic hippocampal neurons. Confocal and total internal reflection fluorescence microscopies demonstrate that this basal localization represents point contacts. Exogenous expression of wild-type Dyn2 and cortactin leads to large, exceptionally flat, and static GCs, whereas disrupting this complex has no such effect. We find that excessive GC spreading is induced by Dyn2 and cortactin over-expression and substantial recruitment of the point contact-associated, actin-binding protein alpha-actinin1 to the ventral GC membrane. The distributions of other point contact proteins such as vinculin or paxillin appear unchanged. Immunoprecipitation experiments show that both Dyn2 and cortactin reside in a complex with alpha-actinin1. These findings provide new insights into the role of Dyn2 and the actin cytoskeleton in GC adhesion and motility.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuron projection morphogenesis involved_inIMP 10047315; 10047315PMID:21210813UniProt 
regulation of axon extension involved_inIMP 10047315; 10047315PMID:21210813UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell junction located_inIDA 10047315; 10047315; 10047315PMID:21210813UniProt 
cytoskeleton located_inIDA 10047315PMID:21210813UniProt 
growth cone located_inIDA 10047315; 10047315PMID:21210813UniProt 
plasma membrane located_inIDA 10047315PMID:21210813UniProt 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Actn1  (actinin, alpha 1)
Cttn  (cortactin)
Dnm2  (dynamin 2)


Additional Information