RGD Reference Report - MicroRNA-145 suppresses ROS-induced Ca2+ overload of cardiomyocytes by targeting CaMKIIdelta. - Rat Genome Database

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MicroRNA-145 suppresses ROS-induced Ca2+ overload of cardiomyocytes by targeting CaMKIIdelta.

Authors: Cha, MJ  Jang, JK  Ham, O  Song, BW  Lee, SY  Lee, CY  Park, JH  Lee, J  Seo, HH  Choi, E  Jeon, WM  Hwang, HJ  Shin, HT  Choi, E  Hwang, KC 
Citation: Cha MJ, etal., Biochem Biophys Res Commun. 2013 Jun 14;435(4):720-6. doi: 10.1016/j.bbrc.2013.05.050. Epub 2013 May 20.
RGD ID: 10047251
Pubmed: PMID:23702479   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2013.05.050   (Journal Full-text)

A change in intracellular free calcium (Ca(2+)) is a common signaling mechanism of reperfusion-induced cardiomyocyte death. Calcium/calmodulin dependent protein kinase II (CaMKII) is a critical regulator of Ca(2+) signaling and mediates signaling pathways responsible for functions in the heart including hypertrophy, apoptosis, arrhythmia, and heart disease. MicroRNAs (miRNA) are involved in the regulation of cell response, including survival, proliferation, apoptosis, and development. However, the roles of miRNAs in Ca(2+)-mediated apoptosis of cardiomyocytes are uncertain. Here, we determined the potential role of miRNA in the regulation of CaMKII dependent apoptosis and explored its underlying mechanism. To determine the potential roles of miRNAs in H2O2-mediated Ca(2+) overload, we selected and tested 6 putative miRNAs that targeted CaMKIIdelta, and showed that miR-145 represses CaMKIIdelta protein expression and Ca(2+) overload. We confirmed CaMKIIdelta as a direct downstream target of miR-145. Furthermore, miR-145 regulates Ca(2+)-related signals and ameliorates apoptosis. This study demonstrates that miR-145 regulates reactive oxygen species (ROS)-induced Ca(2+) overload in cardiomyocytes. Thus, miR-145 affects ROS-mediated gene regulation and cellular injury responses.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cardiac muscle cell apoptotic process involved_inIMP 10047251PMID:23702479BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Camk2d  (calcium/calmodulin-dependent protein kinase II delta)


Additional Information