RGD Reference Report - Differential localization and dynamics of class I myosins in the enterocyte microvillus. - Rat Genome Database

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Differential localization and dynamics of class I myosins in the enterocyte microvillus.

Authors: Benesh, AE  Nambiar, R  McConnell, RE  Mao, S  Tabb, DL  Tyska, MJ 
Citation: Benesh AE, etal., Mol Biol Cell. 2010 Mar 15;21(6):970-8. doi: 10.1091/mbc.E09-07-0638. Epub 2010 Jan 20.
RGD ID: 10047169
Pubmed: PMID:20089841   (View Abstract at PubMed)
PMCID: PMC2836977   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E09-07-0638   (Journal Full-text)

Epithelial cells lining the intestinal tract build an apical array of microvilli known as the brush border. Each microvillus is a cylindrical membrane protrusion that is linked to a supporting actin bundle by myosin-1a (Myo1a). Mice lacking Myo1a demonstrate no overt physiological symptoms, suggesting that other myosins may compensate for the loss of Myo1a in these animals. To investigate changes in the microvillar myosin population that may limit the Myo1a KO phenotype, we performed proteomic analysis on WT and Myo1a KO brush borders. These studies revealed that WT brush borders also contain the short-tailed class I myosin, myosin-1d (Myo1d). Myo1d localizes to the terminal web and striking puncta at the tips of microvilli. In the absence of Myo1a, Myo1d peptide counts increase twofold; this motor also redistributes along the length of microvilli, into compartments normally occupied by Myo1a. FRAP studies demonstrate that Myo1a is less dynamic than Myo1d, providing a mechanistic explanation for the observed differential localization. These data suggest that Myo1d may be the primary compensating class I myosin in the Myo1a KO model; they also suggest that dynamics govern the localization and function of different yet closely related myosins that target common actin structures.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
basolateral plasma membrane located_inIDA 10047169PMID:20089841UniProt 
brush border located_inIDA 10047169PMID:20089841UniProt 
cytoplasmic vesicle located_inIDA 10047169PMID:20089841UniProt 
plasma membrane raft located_inIDA 10047169PMID:20089841UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin filament binding enablesIDA 10047169PMID:20089841UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Myo1d  (myosin ID)


Additional Information