RGD Reference Report - Early cartilage degeneration in a rat experimental model of developmental dysplasia of the hip. - Rat Genome Database

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Early cartilage degeneration in a rat experimental model of developmental dysplasia of the hip.

Authors: Bo, N  Peng, W  Xinghong, P  Ma, R 
Citation: Bo N, etal., Connect Tissue Res. 2012;53(6):513-20. doi: 10.3109/03008207.2012.700346. Epub 2012 Jul 20.
RGD ID: 10043109
Pubmed: PMID:22670655   (View Abstract at PubMed)
DOI: DOI:10.3109/03008207.2012.700346   (Journal Full-text)

Osteoarthritis (OA) is a common long-term complication of developmental dysplasia of the hip (DDH) that is associated with a higher incidence of OA. In addition, the age of onset of OA in DDH patients is significantly younger than in the general population. In order to investigate the early degeneration in DDH cartilage, we used a rat DDH model that was established by the straight-leg swaddling position. The hips were isolated from the DDH model rats and an untreated control group at postnatal weeks 2, 4, 6, and 8. Histology and proteoglycan levels were observed in articular cartilage using Safranin O staining. Biomarkers of cartilage degeneration, including type X collagen and matrix metalloproteinase (MMP)-13, were assessed using immunohistochemistry and quantitative real-time polymerase chain reaction. In addition, expressions of ADAMTS-4 and ADAMTS-5 were studied using quantitative real-time polymerase chain reaction at different ages. DDH rats showed decreased proteoglycans and derangement of chondrocytes when compared with the control group. Collagen X and MMP-13 expressions were higher in the superficial zone of DDH rats than in that of controls (p < 0.05), and the increase was age-dependent. mRNA expression of Collagen X and MMP-13 showed similar results (p < 0.05). A significant increase in mRNA expression of ADAMTS-5 was found in the DDH model cartilage at 8 weeks (p < 0.05). However, no change was observed in ADAMTS-4 expression. This study shows that degenerative cartilage changes occur at an early stage in the rat DDH model and become aggravated with age.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Osteoarthritis, Hip  ISOAdamts5 (Rattus norvegicus)10043109; 10043109mRNA:increased expression:cartilageRGD 
Osteoarthritis, Hip  IEP 10043109mRNA:increased expression:cartilageRGD 
Osteoarthritis, Hip  ISOCol10a1 (Rattus norvegicus)10043109; 10043109mRNA and protein:increased expression:cartilageRGD 
Osteoarthritis, Hip  IEP 10043109; 10043109mRNA and protein:increased expression:cartilageRGD 
Osteoarthritis, Hip  ISOMmp13 (Rattus norvegicus)10043109; 10043109mRNA and protein:increased expression:cartilageRGD 

Objects Annotated

Genes (Rattus norvegicus)
Adamts5  (ADAM metallopeptidase with thrombospondin type 1 motif, 5)
Col10a1  (collagen type X alpha 1 chain)
Mmp13  (matrix metallopeptidase 13)

Genes (Mus musculus)
Adamts5  (ADAM metallopeptidase with thrombospondin type 1 motif 5)
Col10a1  (collagen, type X, alpha 1)
Mmp13  (matrix metallopeptidase 13)

Genes (Homo sapiens)
ADAMTS5  (ADAM metallopeptidase with thrombospondin type 1 motif 5)
COL10A1  (collagen type X alpha 1 chain)
MMP13  (matrix metallopeptidase 13)


Additional Information