RGD Reference Report - Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits. - Rat Genome Database

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Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits.

Authors: O'Donohue, MF  Choesmel, V  Faubladier, M  Fichant, G  Gleizes, PE 
Citation: O'Donohue MF, etal., J Cell Biol. 2010 Sep 6;190(5):853-66. doi: 10.1083/jcb.201005117.
RGD ID: 10002730
Pubmed: PMID:20819938   (View Abstract at PubMed)
PMCID: PMC2935573   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.201005117   (Journal Full-text)

Our knowledge of the functions of metazoan ribosomal proteins in ribosome synthesis remains fragmentary. Using siRNAs, we show that knockdown of 31 of the 32 ribosomal proteins of the human 40S subunit (ribosomal protein of the small subunit [RPS]) strongly affects pre-ribosomal RNA (rRNA) processing, which often correlates with nucleolar chromatin disorganization. 16 RPSs are strictly required for initiating processing of the sequences flanking the 18S rRNA in the pre-rRNA except at the metazoan-specific early cleavage site. The remaining 16 proteins are necessary for progression of the nuclear and cytoplasmic maturation steps and for nuclear export. Distribution of these two subsets of RPSs in the 40S subunit structure argues for a tight dependence of pre-rRNA processing initiation on the folding of both the body and the head of the forming subunit. Interestingly, the functional dichotomy of RPS proteins reported in this study is correlated with the mutation frequency of RPS genes in Diamond-Blackfan anemia.

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ribosome biogenesis pathway   IMP 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730; 10002730 RGD 
ribosome biogenesis pathway   ISOFAU (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   TAS 10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS10 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS11 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS12 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS13 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS14 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS15 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS15A (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS16 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS17 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPS18 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS19 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPS2 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS20 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS21 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS23 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS24 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS25 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS26 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPS27 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPS27A (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS27L (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS28 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS29 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS3 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS3A (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS4X (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS4Y2 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPS5 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS6 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPS7 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS8 (Homo sapiens)10002730; 10002730 RGD 
ribosome biogenesis pathway   ISORPS9 (Homo sapiens)10002730 RGD 
ribosome biogenesis pathway   ISORPSA (Homo sapiens)10002730; 10002730 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Fau  (FAU ubiquitin like and ribosomal protein S30 fusion)
Rps10  (ribosomal protein S10)
Rps11  (ribosomal protein S11)
Rps12  (ribosomal protein S12)
Rps13  (ribosomal protein S13)
Rps14  (ribosomal protein S14)
Rps15  (ribosomal protein S15)
Rps15a  (ribosomal protein S15a)
Rps16  (ribosomal protein S16)
Rps18  (ribosomal protein S18)
Rps19  (ribosomal protein S19)
Rps2  (ribosomal protein S2)
Rps20  (ribosomal protein S20)
Rps21  (ribosomal protein S21)
Rps23  (ribosomal protein S23)
Rps24  (ribosomal protein S24)
Rps25  (ribosomal protein s25)
Rps26  (ribosomal protein S26)
Rps27  (ribosomal protein S27)
Rps27a  (ribosomal protein S27a)
Rps27l  (ribosomal protein S27-like)
Rps28  (ribosomal protein S28)
Rps29  (ribosomal protein S29)
Rps3  (ribosomal protein S3)
Rps3a  (ribosomal protein S3a)
Rps4x  (ribosomal protein S4, X-linked)
Rps4y2  (ribosomal protein S4, Y-linked 2)
Rps5  (ribosomal protein S5)
Rps7  (ribosomal protein S7)
Rps8  (ribosomal protein S8)
Rpsa  (ribosomal protein SA)

Genes (Mus musculus)
Fau  (FAU ubiquitin like and ribosomal protein S30 fusion)
Rps10  (ribosomal protein S10)
Rps11  (ribosomal protein S11)
Rps12  (ribosomal protein S12)
Rps13  (ribosomal protein S13)
Rps14  (ribosomal protein S14)
Rps15  (ribosomal protein S15)
Rps15a  (ribosomal protein S15A)
Rps16  (ribosomal protein S16)
Rps17  (ribosomal protein S17)
Rps18  (ribosomal protein S18)
Rps2  (ribosomal protein S2)
Rps20  (ribosomal protein S20)
Rps21  (ribosomal protein S21)
Rps23  (ribosomal protein S23)
Rps24  (ribosomal protein S24)
Rps25  (ribosomal protein S25)
Rps27a  (ribosomal protein S27A)
Rps27l  (ribosomal protein S27-like)
Rps28  (ribosomal protein S28)
Rps29  (ribosomal protein S29)
Rps3  (ribosomal protein S3)
Rps3a1  (ribosomal protein S3A1)
Rps4x  (ribosomal protein S4, X-linked)
Rps5  (ribosomal protein S5)
Rps6  (ribosomal protein S6)
Rps7  (ribosomal protein S7)
Rps8  (ribosomal protein S8)
Rps9  (ribosomal protein S9)
Rpsa  (ribosomal protein SA)

Genes (Homo sapiens)
FAU  (FAU ubiquitin like and ribosomal protein S30 fusion)
RPS10  (ribosomal protein S10)
RPS11  (ribosomal protein S11)
RPS12  (ribosomal protein S12)
RPS13  (ribosomal protein S13)
RPS14  (ribosomal protein S14)
RPS15  (ribosomal protein S15)
RPS15A  (ribosomal protein S15a)
RPS16  (ribosomal protein S16)
RPS17  (ribosomal protein S17)
RPS18  (ribosomal protein S18)
RPS19  (ribosomal protein S19)
RPS2  (ribosomal protein S2)
RPS20  (ribosomal protein S20)
RPS21  (ribosomal protein S21)
RPS23  (ribosomal protein S23)
RPS24  (ribosomal protein S24)
RPS25  (ribosomal protein S25)
RPS26  (ribosomal protein S26)
RPS27  (ribosomal protein S27)
RPS27A  (ribosomal protein S27a)
RPS27L  (ribosomal protein S27 like)
RPS28  (ribosomal protein S28)
RPS29  (ribosomal protein S29)
RPS3  (ribosomal protein S3)
RPS3A  (ribosomal protein S3A)
RPS4X  (ribosomal protein S4 X-linked)
RPS4Y1  (ribosomal protein S4 Y-linked 1)
RPS4Y2  (ribosomal protein S4 Y-linked 2)
RPS5  (ribosomal protein S5)
RPS6  (ribosomal protein S6)
RPS7  (ribosomal protein S7)
RPS8  (ribosomal protein S8)
RPS9  (ribosomal protein S9)
RPSA  (ribosomal protein SA)


Additional Information