Wdr83os (WD repeat domain 83 opposite strand) - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Gene: Wdr83os (WD repeat domain 83 opposite strand) Rattus norvegicus
Analyze
Symbol: Wdr83os
Name: WD repeat domain 83 opposite strand
RGD ID: 1564058
Description: Predicted to enable protein folding chaperone. Predicted to be involved in multi-pass transmembrane protein insertion into ER membrane. Predicted to be part of multi-pass translocon complex and protein folding chaperone complex. Predicted to be active in endoplasmic reticulum membrane. Orthologous to human WDR83OS (WD repeat domain 83 opposite strand); INTERACTS WITH 2,3,7,8-tetrachlorodibenzodioxine; bis(2-ethylhexyl) phthalate; bisphenol A.
Type: protein-coding (Ensembl: lncRNA)
RefSeq Status: VALIDATED
Previously known as: hypothetical protein LOC288925; LOC288925; PAT complex subunit Asterix; protein Asterix; RGD1564058; similar to cDNA sequence BC056474; uncharacterized protein LOC288925
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Dog
Squirrel
Pig
Green Monkey
Naked Mole-Rat
Alliance Genes
More Info more info ...
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr81939,980,247 - 39,981,619 (-)NCBIGRCr8
mRatBN7.21923,075,373 - 23,076,745 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl1923,075,376 - 23,076,894 (+)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx1929,897,990 - 29,899,362 (-)NCBIRnor_SHRUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.01930,552,354 - 30,553,726 (-)NCBIRnor_SHRSPUTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.01932,775,035 - 32,776,407 (-)NCBIRnor_WKYUTH_Rnor_WKY_Bbb_1.0
Rnor_6.01926,194,336 - 26,195,837 (+)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl1926,194,465 - 26,195,837 (+)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.01937,169,964 - 37,171,460 (+)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.41924,731,659 - 24,733,024 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
Celera1922,633,343 - 22,634,708 (-)NCBICelera
Cytogenetic Map19q11NCBI
JBrowse: View Region in Genome Browser (JBrowse)
Model


Gene Ontology Annotations     Click to see Annotation Detail View

Biological Process

Cellular Component

Molecular Function

References

References - curated
# Reference Title Reference Citation
1. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
2. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
3. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
Additional References at PubMed
PMID:12477932  


Genomics

Comparative Map Data
Wdr83os
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr81939,980,247 - 39,981,619 (-)NCBIGRCr8
mRatBN7.21923,075,373 - 23,076,745 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl1923,075,376 - 23,076,894 (+)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx1929,897,990 - 29,899,362 (-)NCBIRnor_SHRUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.01930,552,354 - 30,553,726 (-)NCBIRnor_SHRSPUTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.01932,775,035 - 32,776,407 (-)NCBIRnor_WKYUTH_Rnor_WKY_Bbb_1.0
Rnor_6.01926,194,336 - 26,195,837 (+)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl1926,194,465 - 26,195,837 (+)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.01937,169,964 - 37,171,460 (+)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.41924,731,659 - 24,733,024 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
Celera1922,633,343 - 22,634,708 (-)NCBICelera
Cytogenetic Map19q11NCBI
WDR83OS
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh381912,668,073 - 12,669,415 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 Ensembl1912,668,073 - 12,669,415 (-)EnsemblGRCh38hg38GRCh38
GRCh371912,778,887 - 12,780,229 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 361912,639,887 - 12,641,449 (-)NCBINCBI36Build 36hg18NCBI36
Celera1912,668,595 - 12,670,179 (-)NCBICelera
Cytogenetic Map19p13.13NCBI
HuRef1912,351,969 - 12,353,553 (-)NCBIHuRef
CHM1_11912,779,386 - 12,780,970 (-)NCBICHM1_1
T2T-CHM13v2.01912,792,571 - 12,793,913 (-)NCBIT2T-CHM13v2.0
Wdr83os
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm39885,807,592 - 85,808,968 (+)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl885,807,369 - 85,808,968 (+)EnsemblGRCm39 Ensembl
GRCm38885,080,963 - 85,082,339 (+)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl885,080,740 - 85,082,339 (+)EnsemblGRCm38mm10GRCm38
MGSCv37887,604,862 - 87,606,238 (+)NCBIGRCm37MGSCv37mm9NCBIm37
MGSCv36887,971,068 - 87,972,444 (+)NCBIMGSCv36mm8
Celera889,375,429 - 89,376,803 (+)NCBICelera
Cytogenetic Map8C3NCBI
cM Map841.55NCBI
Wdr83os
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0NW_00495541531,772,401 - 31,773,916 (-)NCBIChiLan1.0ChiLan1.0
WDR83OS
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
NHGRI_mPanPan1-v22017,596,982 - 17,598,402 (-)NCBINHGRI_mPanPan1-v2
NHGRI_mPanPan11916,597,559 - 16,598,979 (-)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v01912,230,204 - 12,231,881 (-)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.11912,975,314 - 12,976,896 (-)NCBIpanpan1.1PanPan1.1panPan2
PanPan1.1 Ensembl1912,975,314 - 12,976,896 (-)Ensemblpanpan1.1panPan2
WDR83OS
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
CanFam3.12049,443,893 - 49,445,342 (+)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 Ensembl2049,443,951 - 49,445,330 (+)EnsemblCanFam3.1canFam3CanFam3.1
Dog10K_Boxer_Tasha2049,317,346 - 49,318,922 (+)NCBIDog10K_Boxer_Tasha
ROS_Cfam_1.02049,962,443 - 49,964,019 (+)NCBIROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl2049,962,635 - 49,964,014 (+)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.12049,173,239 - 49,174,814 (+)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.02049,599,980 - 49,601,556 (+)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.02049,842,292 - 49,843,868 (+)NCBIUU_Cfam_GSD_1.0
Wdr83os
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2NW_024405118208,558,842 - 208,560,277 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_0049366591,794,432 - 1,795,788 (-)EnsemblSpeTri2.0SpeTri2.0 Ensembl
SpeTri2.0NW_0049366591,794,430 - 1,795,811 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
WDR83OS
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1 Ensembl266,325,992 - 66,328,004 (+)EnsemblSscrofa11.1susScr11Sscrofa11.1
Sscrofa11.1266,312,920 - 66,328,000 (+)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2266,692,103 - 66,705,732 (+)NCBISscrofa10.2Sscrofa10.2susScr3
WDR83OS
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChlSab1.1611,356,542 - 11,358,109 (-)NCBIChlSab1.1ChlSab1.1chlSab2
ChlSab1.1 Ensembl611,356,544 - 11,357,884 (-)EnsemblChlSab1.1ChlSab1.1 EnsemblchlSab2
Vero_WHO_p1.0NW_0236660748,439,378 - 8,440,952 (+)NCBIVero_WHO_p1.0Vero_WHO_p1.0
Wdr83os
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_004624901309,058 - 310,686 (-)EnsemblHetGla_female_1.0HetGla_female_1.0 EnsemblhetGla2
HetGla 1.0NW_004624901309,058 - 310,663 (-)NCBIHetGla_female_1.0HetGla 1.0hetGla2

Variants

.
Variants in Wdr83os
3 total Variants
miRNA Target Status

Predicted Target Of
Summary Value
Count of predictions:114
Count of miRNA genes:95
Interacting mature miRNAs:100
Transcripts:ENSRNOT00000065931
Prediction methods:Microtar, Miranda, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (mRatBN7.2)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
7411590Foco7Food consumption QTL 76.80.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)19124688055Rat
10054132Srcrt9Stress Responsive Cort QTL 92.870.0017blood corticosterone amount (VT:0005345)plasma corticosterone level (CMO:0001173)19127355345Rat
631678Cm9Cardiac mass QTL 94.270.0001aorta mass (VT:0002845)aorta weight (CMO:0000076)19128982497Rat
631681Cm12Cardiac mass QTL 123.330.00053heart left ventricle mass (VT:0007031)heart left ventricle weight to body weight ratio (CMO:0000530)19128982497Rat
1549847Bss8Bone structure and strength QTL 84lumbar vertebra strength trait (VT:0010574)vertebra ultimate force (CMO:0001678)19131963836Rat
9590298Uminl5Urine mineral level QTL 53.590.001urine mineral amount (VT:0015086)urine electrolyte level (CMO:0000593)19136824771Rat
8552935Pigfal10Plasma insulin-like growth factor 1 level QTL 105.7blood insulin-like growth factor amount (VT:0010479)plasma insulin-like growth factor 1 level (CMO:0001299)19136824771Rat
9590250Scort11Serum corticosterone level QTL 1123.450.001blood corticosterone amount (VT:0005345)plasma corticosterone level (CMO:0001173)19136824771Rat
9590090Insglur8Insulin/glucose ratio QTL 810.810.001blood insulin amount (VT:0001560)calculated plasma insulin level (CMO:0002170)19136824771Rat
9589102Slep13Serum leptin concentration QTL 134.630.001blood leptin amount (VT:0005667)plasma leptin level (CMO:0000781)1956937445569374Rat
8694186Bw152Body weight QTL 1523.340.001body mass (VT:0001259)body weight gain (CMO:0000420)1956937445569374Rat
7247442Uae39Urinary albumin excretion QTL 39urine albumin amount (VT:0002871)urine albumin excretion rate (CMO:0000757)19218792746708701Rat
724566Uae12Urinary albumin excretion QTL 125urine albumin amount (VT:0002871)urine albumin level (CMO:0000130)19218792756457239Rat
61447Tcas1Tongue tumor susceptibility QTL 16.08tongue integrity trait (VT:0010553)squamous cell carcinoma of the tongue maximum tumor diameter (CMO:0001875)19231612147316121Rat
2317848Alcrsp21Alcohol response QTL 211.8999999761581420.05response to alcohol trait (VT:0010489)duration of loss of righting reflex (CMO:0002289)19320477748204777Rat
1331737Uae29Urinary albumin excretion QTL 295.5urine albumin amount (VT:0002871)urine albumin level (CMO:0000130)19409615555283277Rat
724518Uae19Urinary albumin excretion QTL 195.5urine albumin amount (VT:0002871)urine albumin excretion rate (CMO:0000757)19745724942983518Rat
724565Tcas5Tongue tumor susceptibility QTL 510.04tongue integrity trait (VT:0010553)number of squamous cell tumors of the tongue with diameter greater than 3 mm (CMO:0001950)19997775339654489Rat
61423Cia14Collagen induced arthritis QTL 143joint integrity trait (VT:0010548)joint inflammation composite score (CMO:0000919)191082797043544039Rat
61407Scl12Serum cholesterol level QTL 120.001blood HDL cholesterol amount (VT:0000184)serum high density lipoprotein cholesterol level (CMO:0000361)191392640130303727Rat
1558656Prcs1Prostate cancer susceptibility QTL 15prostate integrity trait (VT:0010571)percentage of study population developing ventral prostate tumorous lesions during a period of time (CMO:0000943)191511459834521833Rat
7411549Bw130Body weight QTL 13050.001body mass (VT:0001259)body weight gain (CMO:0000420)191545586057337602Rat
1331788Rf45Renal function QTL 452.818kidney blood vessel physiology trait (VT:0100012)absolute change in renal blood flow rate (CMO:0001168)191560502346559041Rat
1578764Stresp19Stress response QTL 193.60.001blood renin amount (VT:0003349)plasma renin activity level (CMO:0000116)191563020157337602Rat
2298478Eau8Experimental allergic uveoretinitis QTL 80.0163uvea integrity trait (VT:0010551)experimental autoimmune uveitis score (CMO:0001504)191715443357337602Rat
61350Bp32Blood pressure QTL 320.012arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)192048357557337602Rat

Markers in Region
MARC_7999-8000:996688236:1  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.21923,075,692 - 23,076,455 (-)MAPPERmRatBN7.2
Rnor_6.01926,194,754 - 26,195,516NCBIRnor6.0
Rnor_5.01937,170,377 - 37,171,139UniSTSRnor5.0
RGSC_v3.41924,731,980 - 24,732,742UniSTSRGSC3.4
Celera1922,633,664 - 22,634,426UniSTS
Cytogenetic Map19q11UniSTS


Expression


RNA-SEQ Expression
High: > 1000 TPM value   Medium: Between 11 and 1000 TPM
Low: Between 0.5 and 10 TPM   Below Cutoff: < 0.5 TPM

alimentary part of gastrointestinal system circulatory system endocrine system exocrine system hemolymphoid system hepatobiliary system integumental system musculoskeletal system nervous system renal system reproductive system respiratory system appendage
High
Medium 3 43 57 41 19 41 8 11 74 35 41 11 8
Low
Below cutoff

Sequence


Reference Sequences
RefSeq Acc Id: ENSRNOT00000065931   ⟹   ENSRNOP00000063158
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.0 Ensembl1926,194,472 - 26,195,837 (+)Ensembl
RefSeq Acc Id: ENSRNOT00000087627   ⟹   ENSRNOP00000070833
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl1923,075,376 - 23,076,894 (+)Ensembl
Rnor_6.0 Ensembl1926,194,465 - 26,195,835 (+)Ensembl
RefSeq Acc Id: NM_001105947   ⟹   NP_001099417
RefSeq Status: VALIDATED
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr81939,980,247 - 39,981,619 (-)NCBI
mRatBN7.21923,075,373 - 23,076,745 (-)NCBI
Rnor_6.01926,194,472 - 26,195,837 (+)NCBI
Rnor_5.01937,169,964 - 37,171,460 (+)NCBI
RGSC_v3.41924,731,659 - 24,733,024 (-)RGD
Celera1922,633,343 - 22,634,708 (-)RGD
Sequence:
RefSeq Acc Id: NM_001399208   ⟹   NP_001386137
RefSeq Status: VALIDATED
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr81939,980,247 - 39,981,619 (-)NCBI
mRatBN7.21923,075,373 - 23,076,745 (-)NCBI
Protein Sequences
Protein RefSeqs NP_001099417 (Get FASTA)   NCBI Sequence Viewer  
  NP_001386137 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein AAI66543 (Get FASTA)   NCBI Sequence Viewer  
  EDL92153 (Get FASTA)   NCBI Sequence Viewer  
  EDL92154 (Get FASTA)   NCBI Sequence Viewer  
Reference Protein Sequences
RefSeq Acc Id: NP_001099417   ⟸   NM_001105947
- Peptide Label: isoform 2
- UniProtKB: A6IY25 (UniProtKB/TrEMBL)
- Sequence:
RefSeq Acc Id: ENSRNOP00000070833   ⟸   ENSRNOT00000087627
RefSeq Acc Id: ENSRNOP00000063158   ⟸   ENSRNOT00000065931
RefSeq Acc Id: NP_001386137   ⟸   NM_001399208
- Peptide Label: isoform 1
- UniProtKB: B2GV64 (UniProtKB/TrEMBL)

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-D3ZYI4-F1-model_v2 AlphaFold D3ZYI4 1-117 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13701042
Promoter ID:EPDNEW_R11565
Type:initiation region
Name:Wdr83os_1
Description:WD repeat domain 83 opposite strand
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.01926,194,478 - 26,194,538EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:1564058 AgrOrtholog
BioCyc Gene G2FUF-6148 BioCyc
Ensembl Genes ENSRNOG00000033611 Ensembl
IMAGE_CLONE IMAGE:7300989 IMAGE-MGC_LOAD
InterPro UPF0139 UniProtKB/TrEMBL
KEGG Report rno:288925 UniProtKB/TrEMBL
MGC_CLONE MGC:188141 IMAGE-MGC_LOAD
NCBI Gene 288925 ENTREZGENE
PANTHER PAT COMPLEX SUBUNIT ASTERIX UniProtKB/TrEMBL
  UPF0139 UniProtKB/TrEMBL
Pfam UPF0139 UniProtKB/TrEMBL
PhenoGen Wdr83os PhenoGen
RatGTEx ENSRNOG00000033611 RatGTEx
UniProt A6IY25 ENTREZGENE, UniProtKB/TrEMBL
  B2GV64 ENTREZGENE, UniProtKB/TrEMBL


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2011-12-06 Wdr83os  WD repeat domain 83 opposite strand  RGD1564058  similar to cDNA sequence BC056474  Nomenclature updated to reflect human and mouse nomenclature 1299863 APPROVED
2008-04-30 RGD1564058  similar to cDNA sequence BC056474   RGD1564058_predicted  similar to cDNA sequence BC056474 (predicted)  'predicted' is removed 2292626 APPROVED
2006-03-07 RGD1564058_predicted  similar to cDNA sequence BC056474 (predicted)  LOC288925  similar to cDNA sequence BC056474  Symbol and Name status set to approved 1299863 APPROVED